FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_DI244_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_DI244_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15384846
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG178500.013.42823580-81
AAAAGGG181250.013.17792178-79
GGGGGGA260500.011.3371882
TAGGGGG64150.010.54420550-51
ACATCTA97350.09.8508384
GGGGGAG257500.09.6423973
AAAAAAG326250.09.56791476-77
CGCCGAT24100.09.4597181
AATCGAG24900.09.3426485
ATCTACA108200.09.0393216
CGGGGAT50550.09.0199491
CCGGGAT46000.08.8796241
TAAAAAA354750.08.86072770-71
GCGTCGT194700.08.69226594-95
CGGCGAT19450.08.5467781
CGCGTAT6706.246755E-58.5066871
CATCTAC116050.08.4274335
CGGAAAT36750.08.2713551
CGGCAAT22600.08.1961551
CGGGCAT47100.08.1680451
Produced by FastQC (version 0.11.5)