FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_DI244_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_DI244_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16366045
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA244450.016.6653842
GGGGGAG243200.013.7237883
GGGGAGA202050.013.1345144
TAGGGGG69000.012.31402150-51
AAAAGGG196250.011.67469278-79
AAGGGGG193350.011.35237580-81
ACATCTA106050.010.8334234
TATAGGG38800.010.5209648-49
AAAGGGG207500.010.36070780-81
CGGGGAT50200.010.1230091
ATCTACA118400.010.0248346
CCGGGAT50600.09.9491251
GGGGGGT213400.09.84136414-15
AAAAAGG258000.09.56215578-79
AGGGGGG267300.09.30957982-83
CATCTAC128200.09.07302955
AAAAAAG361800.08.98292276-77
ACCTTAC46000.08.8762496
CCGGAAT40900.08.8251041
GGGAAAA201600.08.77372622-23
Produced by FastQC (version 0.11.5)