FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_DI222_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_DI222_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762142
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA228000.013.4524172
AAGGGGG171400.011.22646680-81
AAAAGGG183250.011.08935678-79
ACATCTA111450.011.0323484
TAGGGGG67500.010.82918750-51
ACCTTAC52250.010.7218856
CCGGAAT41850.010.32681751
ATCTACA122200.010.217866
AACCTTA63600.010.1518585
GGGGGAG243100.010.07675653
CCGGGAT44850.010.0596241
CATCTAC128350.09.5800475
AATCGAG26300.09.5671785
CGGCGAT20500.09.4984031
CGGAAAT40450.09.2753261
CCCACAT146550.09.1386881
GCCGAAT28050.08.9735361
TACAGAC118600.08.8889149
CGCGAAT10801.1441443E-98.7948171
CGGGAAT36150.08.6707411
Produced by FastQC (version 0.11.5)