FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_DI222_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_DI222_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18891547
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA283900.018.0625042
GGGGGAG281100.014.1023033
GGGGAGA231700.013.440764
TAGGGGG81550.011.84453550-51
ACATCTA123300.011.7816014
AAAGGGG231400.011.62124780-81
AAAAGGG218950.011.53876778-79
AAGGGGG218800.011.02017280-81
ACCTTAC57100.010.7257946
ATCTACA137700.010.5847436
GCGGAAT26050.010.3921441
CCCACAT161500.010.3516161
AGGGGGG306200.010.27502982-83
GGGGGGT248850.010.11045214-15
GTCGGTT31050.010.0953261
AAAAAGG297950.010.06669878-79
TAGCGAT24300.09.7712999
GAGCGGG105450.09.7273779
CATCTAC143350.09.6372435
GTTCGAT27700.09.601661
Produced by FastQC (version 0.11.5)