FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_DI222_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_DI222_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14833673
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA172450.014.7852072
CGGGCAT45050.010.6478051
TAGGGGG54200.010.24610250-51
CGGCGAT19600.010.1771631
GGGGGAG192700.010.1503063
ACATCTA97550.09.9764734
CGACGAT17850.09.8445761
CCGGTTT33150.09.7422421
ACCTTAC47250.09.445216
CGGCAAT22750.09.3943041
CCGGGAT44300.09.3271531
ATCTACA107600.09.2660076
GGGGAGA171750.09.0662484
CGCGGAT15750.09.0463681
AAGGGGG134250.08.93624380-81
AAAAGGG143900.08.7045978-79
CATCTAC114350.08.5110725
CTCGAAT33000.08.491251
TCGTGTA128950.08.44875694-95
AACCTTA57600.08.4070635
Produced by FastQC (version 0.11.5)