FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n02_KO_DI222_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n02_KO_DI222_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17514969
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGGA225400.015.0405872
CGGGGAT55650.011.3503991
ACATCTA111250.011.2657454
GGGGGAG249700.010.7422643
AATCGAG28400.010.1972055
AAAAGGG182850.09.9433178-79
ATCTACA129200.09.8483696
GGGGAGA208950.09.7924394
CGGGAAT37000.09.7552091
AAGGGGG178450.09.74443680-81
TAGGGGG67250.09.74019950-51
CCCACAT147150.09.6501851
ACCTTAC50800.09.3460546
CCGGGAT53700.09.2862361
CATCTAC137400.09.0528275
AAAGGGG191450.09.02072180-81
TACAGAC123400.09.0052999
CGCGAAT11655.638867E-118.96853351
CGCGGAT18350.08.7996931
CGCGTAA4950.0026583588.6349941
Produced by FastQC (version 0.11.5)