FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34177597
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG382241211.183969428863007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC241900.034.11838
CGGAAGA288400.029.448384
TCGGAAG294800.028.872583
ATCGGAA296150.028.7410932
GATCGGA300850.027.8174481
CTCGTAT53050.027.58768344-45
TCTCGTA57200.027.21264542-43
CGTCTGA151550.026.89873516-17
ATGCCGT62850.026.76787648-49
ACGTCTG156150.026.09173414-15
CACGTCT160500.025.12030614-15
ACACGTC161700.024.96638912-13
GAACTCC163150.024.20574620-21
CACACGT168900.023.9300112-13
CTGAACT181950.022.55920618-19
ATCTCGT63300.022.46655342-43
TGAACTC178300.022.4397420-21
GCACACG180850.022.28495610-11
ACTCGAT24250.021.57295236-37
CAGTCAC140400.020.8790426-27
Produced by FastQC (version 0.11.5)