FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_PR8_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_PR8_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15584961
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG29700.035.08895540-41
ATCGCGT22900.031.51357742-43
CGCGTAT17450.029.52046444-45
TCGCGTA18900.028.2605342-43
CACTCCG135250.026.15167430-31
GAATCGC44200.024.97428540-41
ACTCCGG143500.024.79746232-33
AGAATCG77400.024.41371238-39
TATGCCG33250.024.2750548-49
CCGGAGA159450.022.12350334-35
TCCGGAG165650.021.53907834-35
CTCCGGA168600.021.38738632-33
CGGAGAA166100.021.25212336-37
AATCTCG50700.020.6018940-41
CGTATGC33350.020.35719546-47
GAGAATC169400.019.89924638-39
CTCGTAT31250.019.75068744-45
GTCACTC186350.019.2223728-29
AATCGGG37800.019.21728340-41
TCACTCC188800.018.96050530-31
Produced by FastQC (version 0.11.5)