FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_PR8_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_PR8_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17203756
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT902070.5243448000541278TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG126400.040.5289448-49
CGTATGC131500.038.9896946-47
TCGTATG131300.038.56118844-45
CTCGTAT125050.038.36249544-45
TACTCGA135350.037.5476436-37
ACTCGAT133500.036.2543636-37
TCTCGTA132650.034.66159442-43
ATCTCGT137950.033.29549442-43
ATGCCGT153350.033.15862748-49
TTACTCG158950.032.31620834-35
CTCGATC144450.032.04369738-39
GCCGTCT159300.031.73031850-51
CCGTCTT160650.031.69470852-53
TGCCGTC162250.031.56312650-51
TCGATCT144850.031.5291438-39
CGATCTC147450.031.34362640-41
GTATGCC164600.031.07700746-47
GATCTCG152050.030.06754140-41
ACATTAC176800.029.29513732-33
ATTACTC182650.028.42187734-35
Produced by FastQC (version 0.11.5)