FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_PR8_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_PR8_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15792513
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT461170.2920181227648823TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG73150.035.6990648-49
TCGTATG75300.034.64582444-45
CGTATGC76100.034.56234746-47
CTCGTAT74250.033.31343544-45
TCTCGTA80450.030.03796842-43
CGAGATT95100.027.9816932-33
ATCTCGT86650.027.61473342-43
ATGCCGT96750.027.23644648-49
GTCACGA100600.026.7582128-29
TCACGAG103750.026.01467730-31
TGCCGTC106450.024.89147250-51
CCGTCTT107050.024.80173552-53
AGTCACG108450.024.79946728-29
GCCGTCT107500.024.64834450-51
ACGAGAT108650.024.62311732-33
GTATGCC111600.024.03601346-47
CACGAGA114600.023.40668530-31
CATCTCG110800.021.57459640-41
CGTCTTC138750.019.30647952-53
GAGATTC144750.018.62987734-35
Produced by FastQC (version 0.11.5)