FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_PR8_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_PR8_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16747055
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT871000.5200914429432518TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG108000.039.9396948-49
CTCGTAT111550.037.6443144-45
TCGTATG114250.037.6065544-45
CGTATGC115450.037.35947846-47
TATCTCG111550.037.1548740-41
TCTCGTA121200.034.2161742-43
ATGCCGT132550.032.52442648-49
ATCTCGT130350.032.1057242-43
TGCCGTC140100.030.860650-51
GCCGTCT140700.030.34087850-51
ACCGCTC144400.029.83668332-33
GTATGCC145200.029.8356946-47
CGCTCAT145500.029.6274334-35
CCGTCTT148450.029.00356152-53
TCACCGC157000.027.24567430-31
CACCGCT160550.026.74672930-31
CCGCTCA162200.026.56237432-33
CATTATC168650.025.22280538-39
GTCACCG171350.025.21330328-29
TTATCTC170950.024.77233140-41
Produced by FastQC (version 0.11.5)