FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_DI222_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_DI222_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15232146
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT157750.1035638707769739TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG32800.039.0103840-41
ATCGCGT25200.035.42026542-43
CGCGTAT19700.033.37903644-45
TCGCGTA20100.033.30562642-43
CACTCCG144600.030.17372530-31
TATGCCG37750.028.73932848-49
ACTCCGG152650.028.70706232-33
GAATCGC47100.027.67048340-41
AGAATCG86550.026.3307838-39
CCGGAGA168950.026.02175734-35
TCCGGAG174750.025.3890234-35
CTCCGGA176400.025.08425132-33
CGGAGAA179350.024.39386736-37
CGTATGC37250.023.51517746-47
GAGAATC176950.022.83329238-39
AATCTCG51850.022.80057740-41
CTCGTAT34050.022.79769344-45
GTCACTC193550.022.70196728-29
TCACTCC196500.022.37330430-31
AATCGGG42850.021.32922740-41
Produced by FastQC (version 0.11.5)