FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_DI222_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_DI222_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15310275
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT837370.5469333503153928TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG112900.040.7962448-49
CGTATGC116950.039.56345446-47
TCGTATG117150.039.33379444-45
CTCGTAT112250.038.87243344-45
TACTCGA122050.037.34616536-37
ACTCGAT119050.036.49255836-37
TCTCGTA117350.035.7466542-43
TTACTCG136000.033.9691834-35
ATGCCGT136750.033.61169448-49
ATCTCGT124300.033.3850642-43
CGATCTC129450.032.0203640-41
TCGATCT129100.031.9783438-39
GCCGTCT144300.031.9389450-51
TGCCGTC144500.031.89473350-51
CTCGATC130750.031.79267338-39
GTATGCC150100.031.07881446-47
CCGTCTT150650.030.64603452-53
GATCTCG134800.030.59101940-41
ACATTAC160900.029.0073932-33
ATTACTC160700.028.95485334-35
Produced by FastQC (version 0.11.5)