FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_DI222_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_DI222_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16212232
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT850990.5249061326040733TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG118650.040.39727848-49
TCGTATG121450.039.3073144-45
CTCGTAT116750.039.08006744-45
CGTATGC123400.038.93612746-47
TCTCGTA123400.035.18519642-43
ATGCCGT141150.033.70546748-49
CGAGATT144750.033.35783432-33
ATCTCGT132150.032.85549542-43
TCACGAG155050.031.32549730-31
GCCGTCT152500.031.20055450-51
TGCCGTC153650.031.13701250-51
GTCACGA155400.031.08681128-29
GTATGCC156100.030.84057446-47
CCGTCTT159800.029.95978452-53
ACGAGAT162100.029.8606832-33
AGTCACG165150.029.4527728-29
CACGAGA165550.029.41037830-31
CATCTCG155400.027.81773240-41
CGTCTTC191350.025.0323952-53
GAGATTC195600.024.91630234-35
Produced by FastQC (version 0.11.5)