FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_WT_DI222_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_WT_DI222_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17865726
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1213170.6790488111146449TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG152450.041.34316348-49
TCGTATG159000.039.78716744-45
CTCGTAT157550.039.39999844-45
CGTATGC160700.039.2918646-47
TATCTCG162000.037.99587640-41
TCTCGTA167150.036.91020642-43
ATGCCGT179250.035.13538448-49
ATCTCGT177000.034.92321842-43
TGCCGTC188250.033.57346750-51
CCGTCTT191250.033.10389352-53
GCCGTCT191350.032.89306650-51
GTATGCC193800.032.6545146-47
CGCTCAT209450.030.49834434-35
ACCGCTC212900.030.0932832-33
CATTATC217950.028.57954438-39
TTATCTC218700.028.45986640-41
CGTCTTC225400.028.28855752-53
CCGCTCA227550.028.18712632-33
CTCATTA232650.027.60018336-37
ATTATCT228400.027.28233538-39
Produced by FastQC (version 0.11.5)