FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_PR8_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_PR8_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19345033
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT217120.11223552836534319TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG43450.038.62584740-41
ATCGCGT34750.033.95062342-43
CGCGTAT26100.033.560644-45
TCGCGTA28050.031.56618742-43
CACTCCG188750.031.10139730-31
TATGCCG48800.030.1607548-49
ACTCCGG202200.029.22057332-33
AGAATCG109800.027.78104838-39
GAATCGC63550.027.60433240-41
CCGGAGA215950.027.21724134-35
TCCGGAG225400.026.36049834-35
CTCCGGA228100.025.97552332-33
CGGAGAA231400.025.38988936-37
GAGCACA497250.024.2316119
CGTATGC50750.023.76131246-47
GTCACTC249900.023.7568328-29
CTCGTAT45650.023.60798644-45
AATCGGG51200.023.5991440-41
TCACTCC253650.023.52735930-31
GAGAATC237000.023.31752438-39
Produced by FastQC (version 0.11.5)