FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_PR8_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_PR8_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18839539
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT842180.44702792356012533TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG121400.038.9729648-49
TCGTATG126550.037.53571744-45
CTCGTAT117850.037.36578444-45
CGTATGC128350.036.93459346-47
TACTCGA130200.036.2281436-37
ACTCGAT127200.035.58957736-37
TTACTCG141600.033.8645734-35
TCTCGTA127900.033.5762242-43
ATGCCGT151400.031.56404748-49
ATCTCGT137350.030.62660642-43
TGCCGTC159250.030.0570550-51
GCCGTCT158850.029.92348350-51
TCGATCT142250.029.88870638-39
CCGTCTT161100.029.3054552-53
CTCGATC145400.029.01261338-39
CGATCTC146350.028.95412440-41
GTATGCC166150.028.91753646-47
GATCTCG147500.028.85713840-41
ATTACTC170000.028.360834-35
ACATTAC173450.027.9061832-33
Produced by FastQC (version 0.11.5)