FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_PR8_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_PR8_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18119005
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT796000.4393177219168492TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG112900.039.86643248-49
CTCGTAT114050.038.02645544-45
TCGTATG118000.037.94043444-45
CGTATGC120200.037.56152746-47
TCTCGTA119800.034.7946242-43
ATGCCGT137950.032.455148-49
CGAGATT142100.031.78954732-33
ATCTCGT133700.031.2482542-43
TCACGAG150400.030.4612230-31
GTCACGA148850.030.42749228-29
TGCCGTC152050.029.82456250-51
GCCGTCT150200.029.63865350-51
ACGAGAT154300.029.61450232-33
CCGTCTT153650.029.38241852-53
AGTCACG157050.029.24688728-29
GTATGCC156150.028.94424446-47
CACGAGA161900.028.15089430-31
CATCTCG160700.025.865240-41
CGTCTTC191700.023.62471652-53
GAGATTC198300.023.15119434-35
Produced by FastQC (version 0.11.5)