FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_PR8_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_PR8_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18396546
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1007640.5477332538401501TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG129100.040.26988248-49
CTCGTAT132300.038.82711444-45
TCGTATG136150.038.4091444-45
TATCTCG132800.038.2521440-41
CGTATGC137250.038.101246-47
TCTCGTA142800.035.77280442-43
ATGCCGT152850.034.059348-49
ATCTCGT152450.033.69525542-43
ACCGCTC160100.032.678532-33
GCCGTCT160400.032.44543550-51
TGCCGTC164450.031.87737850-51
CGCTCAT166550.031.47009734-35
CCGTCTT166550.031.29769752-53
GTATGCC173000.030.25513346-47
TCACCGC175100.030.02784230-31
CCGCTCA179600.029.18331332-33
CACCGCT181800.028.86897930-31
GTCACCG187050.028.07127828-29
CATTATC191450.027.04209938-39
TTATCTC197000.026.07540740-41
Produced by FastQC (version 0.11.5)