FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_DI244_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_DI244_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15384846
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1073910.6980310365147626TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG138250.041.02340348-49
TCGTATG143700.039.364944-45
CTCGTAT134550.039.3249544-45
CGTATGC144250.039.24733746-47
AATCTCG142350.037.0705340-41
TCTCGTA143800.036.9112142-43
ATGCCGT161050.035.48101448-49
ATCTCGT152300.034.6017842-43
TGCCGTC169400.033.48463450-51
GCCGTCT170100.033.33287450-51
GTATGCC173850.032.96098346-47
CCGTCTT172600.032.81552552-53
CGTCTTC204450.027.85439952-53
GCTTGAA211450.027.02049460-61
AGGGGGG186300.026.76249974-75
TGCTTGA219900.025.79845660-61
AAATCTC211850.025.13318840-41
GTCACAT235700.024.80514328-29
GAGCACA479550.024.6011359
AAGGGGG198550.024.35726774-75
Produced by FastQC (version 0.11.5)