FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_DI244_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_DI244_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16366045
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT1162830.7105137496566825TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG145750.041.6648948-49
TCGTATG150450.040.3601544-45
CTCGTAT143300.040.1707544-45
CGTATGC152800.039.81691746-47
TCTCGTA154050.037.2906642-43
AATCTCG157100.036.50635540-41
ATCTCGT160100.035.7035642-43
ATGCCGT170600.035.6932948-49
TGCCGTC179300.033.8475450-51
GCCGTCT179750.033.7363950-51
CCGTCTT180250.033.439352-53
GTATGCC186150.033.02776346-47
CGTCTTC216500.028.09263852-53
GCTTGAA229900.026.7428160-61
AAATCTC221100.026.21830640-41
AGGGGGG208950.025.93085374-75
GAGCACA489900.025.8162169
TGCTTGA240100.025.49783160-61
AGAGCAC501600.025.1004668
CTTGAAA244800.025.05568162-63
Produced by FastQC (version 0.11.5)