FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_DI222_M2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_DI222_M2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16762142
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTAT187640.1119427338105118TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG38600.038.25245340-41
ATCGCGT29550.036.1499242-43
CGCGTAT24300.033.60479744-45
TCGCGTA25400.032.33662842-43
CACTCCG168850.029.59353830-31
TATGCCG42450.029.08024448-49
ACTCCGG178900.028.0373932-33
GAATCGC55000.027.83897840-41
AGAATCG99250.027.24261338-39
CCGGAGA190150.026.32869534-35
TCCGGAG204100.024.77340534-35
AATCGGG44050.024.62785140-41
CTCGTAT39150.024.3749344-45
CGGAGAA206100.024.36052536-37
CTCCGGA207700.024.32112132-33
AATCTCG61350.023.99017140-41
GAGAATC200900.023.60864838-39
GTCACTC221550.022.88625328-29
CGTATGC47100.022.62907246-47
TCACTCC228300.022.16803230-31
Produced by FastQC (version 0.11.5)