FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_DI222_M1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_DI222_M1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18891547
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT1316590.6969201622291705TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG176450.042.178448-49
CTCGTAT172800.040.57974244-45
TCGTATG185400.040.15215744-45
CGTATGC187250.039.88170246-47
TACTCGA186300.039.42287436-37
ACTCGAT181600.038.82231536-37
TCTCGTA180700.037.6365842-43
ATGCCGT204500.036.32339548-49
TTACTCG208150.036.16258634-35
ATCTCGT186950.035.69267742-43
GCCGTCT212100.035.04905750-51
TGCCGTC212750.034.97545650-51
TCGATCT191800.034.7653738-39
CGATCTC193150.034.5348140-41
CTCGATC194700.034.41823638-39
GTATGCC219300.034.00982346-47
CCGTCTT219600.033.7955252-53
GATCTCG200900.033.34436840-41
ATTACTC236000.031.9353334-35
ACATTAC238000.031.66684232-33
Produced by FastQC (version 0.11.5)