FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_DI222_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_DI222_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14833673
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT657250.4430797416122089TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG87350.039.1091948-49
TCGTATG92800.036.91295644-45
CTCGTAT89650.036.91245744-45
CGTATGC95300.036.09346846-47
TCTCGTA94100.033.8804442-43
CGAGATT109250.031.72380832-33
ATCTCGT102100.031.48151242-43
ATGCCGT108800.031.35514648-49
GTCACGA120050.028.86936828-29
GCCGTCT119100.028.78651850-51
GTATGCC119700.028.71624246-47
TGCCGTC120000.028.66954650-51
ACGAGAT123600.028.21351632-33
CCGTCTT122350.028.06705552-53
TCACGAG124650.028.05184630-31
AGTCACG125550.027.75593828-29
CACGAGA129150.027.00090830-31
CATCTCG129000.024.7696740-41
CGTCTTC151850.022.88031852-53
GAGATTC157400.022.26053234-35
Produced by FastQC (version 0.11.5)