FastQCFastQC Report
Thu 31 Jan 2019
HH5H2BGX9_n01_KO_DI222_F1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5H2BGX9_n01_KO_DI222_F1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17514969
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT1021340.5831240694745163TruSeq Adapter, Index 2 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG127700.039.8017548-49
TATCTCG128200.038.51506440-41
CTCGTAT129900.038.21171644-45
TCGTATG132900.038.18865244-45
CGTATGC134750.037.7696846-47
TCTCGTA141250.035.0910342-43
ATGCCGT154300.032.92488548-49
ATCTCGT151550.032.8470742-43
TGCCGTC161800.031.49136450-51
GCCGTCT161150.031.44158250-51
ACCGCTC162200.031.31939132-33
CGCTCAT167050.030.63749734-35
CCGTCTT166350.030.62360852-53
GTATGCC167100.030.44338846-47
TCACCGC178300.028.61091430-31
CCGCTCA181600.028.0781732-33
CACCGCT182300.027.86594430-31
CATTATC183350.027.24074238-39
TTATCTC183700.027.11138340-41
GTCACCG192400.026.45225128-29
Produced by FastQC (version 0.11.5)