FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepmh-1_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepmh-1_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14871243
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC304830.20497950305835227TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG51300.056.15246646
CGTATGC57700.050.4100844
GTATGCC60400.047.98231545
ATGCCGT62250.046.1618147
CTAATCG64550.045.3812233
ACGTCTG68150.043.60037615
TGCCGTC66850.043.2470348
TCGTATG66800.042.91449743
GCACACG71600.041.89084211
GCCGTCT73850.039.14659549
CACACGT76250.038.9229612
ACTAATC79550.037.13232432
ACACGTC80400.036.8704713
CTCGTAT65050.036.58952342
CACTAAT83300.035.67089531
GTCACTA88150.033.6292229
AGTCACT89700.033.39938428
CACGTCT89900.033.24666214
CGTCTGA90050.032.91911316
AGCACAC95050.032.10817710
Produced by FastQC (version 0.11.2)