FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepmh-1_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepmh-1_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25353007
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46250.046.01180346
CGTATGC55800.038.38875644
GTATGCC59250.036.2714745
ATGCCGT65750.032.47205447
TCGTATG68500.031.11837843
ACGTCTG79250.028.70565815
GTCACGT80100.027.83323929
GCACACG83000.027.19766411
TGCCGTC79000.027.07004248
TCACGTA83650.026.5264630
CACGTAG85150.025.64819131
CACACGT91600.025.14092412
AGTCACG91100.024.5877328
ACACGTC96250.023.7809713
GCCGTCT91200.023.4482349
CTCGTAT81550.023.2199842
CGTAGAG106450.020.81188633
ACGTAGA109250.020.34260632
AGCACAC114250.020.27924310
CGTCTGA116350.020.18410716
Produced by FastQC (version 0.11.2)