FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepmh-1_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepmh-1_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13525377
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14350.026.83025746
ACGTCTG36200.020.40032415
GCACACG37250.020.20126311
GTATGCC20650.018.9838945
CACACGT40000.018.81228812
CGTATGC20200.018.71376844
ATGCCGT24750.015.98009247
TGCCGTC27250.015.02779948
ACACGTC51800.014.66199913
CACGTCT52900.014.22479414
CGTCTGA54700.014.01264116
TCGTATG28650.013.56100943
GCGGGGA8950.013.2979561
AGCACAC60150.013.20825210
GGGGCGC6500.012.92288429
CGCGTAT11950.012.595911
GAGCACA66350.012.18501959
CTCGTAT32700.011.88147942
GCCGAAT19000.011.6068931
GGCGAAT29950.011.4540291
Produced by FastQC (version 0.11.2)