FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepkn-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepkn-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15323401
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC350540.22876122604896915TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG62500.057.625446
GTATGCC69350.052.2369945
CGTATGC69400.052.04839744
ATGCCGT70800.050.86971747
GCACACG82850.046.3416511
TCGTATG78100.046.2505843
ACGTCTG83400.045.9937715
TGCCGTC79750.044.8536148
GCCGTCT82600.042.96588549
CACACGT89750.042.85688412
ACACGTC92800.041.3351913
CTCGTAT73800.039.7914642
CACGTCT100350.038.2252714
CCAACTA97550.038.2098633
CGTCTGA103750.037.1410116
AGCACAC106400.036.5778110
AACTAAT98550.036.1896935
TCGGAAG108050.035.7315673
ACTAATC81550.035.19341336
GAGCACA114000.034.538519
Produced by FastQC (version 0.11.2)