FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepkn-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepkn-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27368379
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG56400.049.6471846
CCCGTCC76500.046.6643433
CACCCGT86200.041.6976931
CGTATGC67400.041.59706544
ACCCGTC89350.040.6584532
TCACCCG91850.039.4756630
GTATGCC74350.038.32021345
GTCACCC96050.037.5310129
ACGTCTG105300.036.69386715
GCACACG108650.035.82057611
ATGCCGT76600.035.54947
CACACGT114650.034.00665312
TCGTATG80950.032.86187443
ACACGTC125800.030.90908613
TGCCGTC90150.028.96340848
CGTCTGA137000.028.33106816
CCGTCCA126700.027.12626834
CACGTCT150050.025.93701414
GAGCACA157500.025.3548939
AGCACAC155950.025.27021610
Produced by FastQC (version 0.11.2)