FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepkn-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepkn-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14784815
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC372400.25188005396076985TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67150.059.16015246
GTATGCC73550.054.34559245
CGTATGC73450.053.27609644
GCACACG108400.052.07792311
ACGTCTG108100.052.0283715
ATGCCGT75800.050.93089747
CACACGT111400.050.7381312
ACACGTC116850.048.2821213
TCGTATG74650.047.965943
TGCCGTC80900.047.20084448
CGTCTGA124700.045.2146416
CACGTCT127700.044.09762614
GCCGTCT80150.043.40534249
TCGGAAG135150.042.0111123
GAGCACA136000.041.8695879
AGCACAC136550.041.777710
CTCGTAT75350.039.99495342
GATCGGA141100.039.89481
ATCGGAA147500.038.4459762
TCCAGTC151950.036.99214625
Produced by FastQC (version 0.11.2)