FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepge-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepge-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18159842
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25200.040.41800746
GTATGCC35400.029.16787745
CGTATGC35300.029.15159244
ATGCCGT37800.027.31519547
TCGTATG44750.022.9175343
ACGTCTG47700.022.8187715
GCACACG48100.022.33832611
TGCCGTC45850.022.2140548
CACACGT50450.021.71385612
GCCGTCT51700.019.09080749
ACACGTC58050.018.62987913
CTCGTAT51500.017.19512442
CGTCTGA71900.015.381816
AGCACAC78250.014.89420210
CACGTCT73450.014.86672714
GAGCACA79650.014.7642339
TCGGAAG84200.013.178163
GCGGGAT26100.013.0104951
GCGGACT15250.011.9370241
GGGCGCT4802.7066562E-911.6692071
Produced by FastQC (version 0.11.2)