FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepge-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepge-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17230340
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25150.038.826748
ACGTCTG56600.032.03096815
GCACACG58600.031.4154711
GTATGCC33000.030.22700947
CGTATGC33850.029.77852246
CACACGT63750.029.09702712
ACACGTC67100.027.01858913
ATGCCGT36550.026.81180849
TCGTATG40700.025.11085745
CGTCTGA79350.023.15629416
CACGTCT80950.022.7418414
ATGTCAG77550.022.70062434
AGCACAC89150.021.23862310
GTCAGAA82900.021.1097736
GAGCACA93000.020.9238829
ACATGTC91200.020.1470932
TGCCGTC46050.020.14050750
TGTCAGA89400.019.92700435
TCGGAAG93800.019.7032663
GTCACAT92150.019.33193629
Produced by FastQC (version 0.11.2)