FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepge-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepge-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13640917
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC148730.10903225934150909TruSeq Adapter, Index 6 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG26850.046.6675646
GTCACGC31300.042.37898329
CGTATGC32950.039.40938644
GTATGCC35050.036.64861745
TCACGCC37300.036.2188130
ATGCCGT35600.035.39354747
ACGTCTG42300.032.26846715
TCGTATG41700.031.3924143
CACGCCA43900.031.25195931
TGCCGTC42800.029.68471548
GCACACG46800.029.4650811
CACACGT48250.028.57918212
GCCGTCT46250.026.86459249
CTCGTAT46400.026.77941342
TATCTCG47350.026.3899739
ACGCCAA52350.026.20727532
CGCCAAT52900.025.73603633
ACACGTC53850.025.41227313
AGTCACG54950.024.52150528
CGTCTGA64650.021.32947516
Produced by FastQC (version 0.11.2)