FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepct-1_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepct-1_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16773392
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG21050.033.4210248
CGTATGC29400.024.76249146
GTATGCC29850.023.92009447
ATGCCGT32350.022.07089449
ACGTCTG40100.019.89959315
TCGTATG37400.019.6529345
GCACACG44450.017.40120911
TGCCGTC41550.016.84671850
TATCTCG42800.016.51957541
CACACGT49450.016.06644412
CACACTG51700.015.1639131
CTCGTAT47300.015.09571944
ACACGTC54750.014.7029313
GCCGTCT50200.014.36233351
CACGTCT65500.012.50355314
CGTCTGA65300.012.16652116
GCGGGGT6050.012.1515111
AGCACAC69800.012.13445110
GCCGGAT31750.012.128731
GCGGGCC3802.1557571E-711.9763951
Produced by FastQC (version 0.11.2)