FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepct-1_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepct-1_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26146282
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT342410.13095934634224476TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60750.048.5685648
CGTATGC70550.041.52533346
GTATGCC74900.039.72023847
ACGTCTG95850.036.8794915
ATGCCGT82000.036.1092849
TCGTATG79100.035.0014845
GCACACG104700.034.06295811
TCCGTAT96000.033.68911737
CACACGT110000.032.26259212
GTTCCGT104000.032.07187735
TGCCGTC95350.030.68630250
ACACGTC117550.030.10128213
CGTATCT90350.029.83061839
TTCCGTA111450.029.77148836
TATCTCG89950.029.57436441
CTCGTAT93350.027.74664144
GCCGTCT101400.027.37111951
AGTTCCG122500.027.25610534
CCGTATC107750.027.22234738
CGTCTGA136550.026.27152816
Produced by FastQC (version 0.11.2)