FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepct-1_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepct-1_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14411721
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32000.047.57917446
CGTATGC36600.041.88762744
ATGCCGT40950.037.17966547
ACGTCTG53150.036.74421715
GTATGCC42850.036.1855445
GCACACG57450.034.05503511
CACACGT58100.033.613812
TCGTATG45200.032.44698343
ACACGTC65050.030.18398713
TGCCGTC49450.029.868848
CACAGTG70450.026.9754933
CGTCTGA73400.026.84533916
CACGTCT75350.026.15087314
GCCGTCT52800.026.11691549
CACACAG76600.025.12953631
AGCACAC79750.024.8831810
ACACAGT79100.024.335332
TCGGAAG83400.023.6283593
GAGCACA86150.023.4002639
CTCGTAT39750.020.95738442
Produced by FastQC (version 0.11.2)