FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepcol-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepcol-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13887877
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG16600.033.7360746
CGTATGC23650.022.9399544
GTATGCC26500.021.39717145
ACGTCTG33150.020.1649915
ATGCCGT28500.019.52675847
GCACACG37300.018.10896111
TGCCGTC32850.017.89988348
TCGTATG31300.017.5571343
GCCGTCT35900.016.67114649
ACGGTAG39600.016.6156432
CACACGT43700.015.69710912
AGTCACG44200.014.64875628
ACACGTC46300.014.588860513
GTCACGG46550.014.51082229
CTCGTAT36350.014.25128242
CACGTCT53900.012.85650814
GGTAGCA52050.012.84329534
CGTCTGA53300.012.67296616
GAGCACA62000.012.5882689
CGGTAGC57250.012.41013533
Produced by FastQC (version 0.11.2)