FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepcol-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepcol-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25294159
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT394420.1559332334393881TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66350.051.85372548
GTATGCC78000.044.28856747
CGTATGC78900.044.1831446
ATGCCGT86200.040.3996949
TCGTATG91000.037.88530345
ACGTCTG99450.037.37439715
TGCCGTC95500.035.91567650
GCACACG105100.035.2658711
CTCGTAT90500.034.150444
GCCGTCT105900.032.2231751
CACACGT115700.032.1860712
ACACGTC120950.030.64430213
CACGTCT131800.028.17455314
AGCACAC134800.028.1446310
CACAGTC135700.026.79729831
ACAGTCA136250.026.76597832
CGTCTGA142050.026.36318416
GAGCACA147100.026.0768899
TCGGAAG149100.025.0963883
GTCACAG158050.023.14082529
Produced by FastQC (version 0.11.2)