FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepcol-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepcol-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14290325
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC151660.10612774726956875TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30600.051.2430546
CGTATGC38550.041.0387444
GTATGCC40150.039.3158745
ATGCCGT41250.037.84293447
TGCCGTC45150.034.80654548
ACGTCTG49150.034.2508315
TCGTATG48850.032.6724543
GCACACG52800.031.88344811
ACTGACC54200.029.76841732
GCCGTCT52550.029.438249
CACTGAC55250.029.13923531
CACACGT58850.028.90286312
ACACGTC58500.028.4776213
CTCGTAT49550.025.5709242
CGTCTGA70650.023.97633416
AGTCACT69500.023.86982228
CACGTCT71050.023.6444814
AGCACAC76250.022.58296210
GAGCACA78000.022.5249969
TCGGAAG75750.022.1341673
Produced by FastQC (version 0.11.2)