FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepbur-0_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepbur-0_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7268164
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCAAAG352580.48510187717283215No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCA268300.3691441194777663No Hit
CGGCAATCGGGCGGCGGGCGCATGCGTCGCTTCTAGCCCGGATTCTGACT231640.31870497143432647No Hit
GAACAATCCAACACTTGGTGAATTCTGCTTCACAATGATAGGAAGAGCCG202820.27905259154856715No Hit
CACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCTT177270.24389928460612612No Hit
CCCGACTGTCCCTGTTAATCATTACTCCGATCCCGAAGGCCAACACAATA174040.23945524619422456No Hit
GCACTCTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTA173620.2388773836143488No Hit
CCGGAACCCAAAAACTTTGATTTCTCATAAGGTGCCAGCGGAGTCCTATA151330.2082093909823719No Hit
GCGGTTATGAGTACGACCGGGCGTGAGCGGCACTCGGTCCTCCGGATTTT148590.20443952558032535No Hit
GTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAGC146700.20183914397088454No Hit
CTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTC125540.17272587685143043No Hit
GCAGAAATCACATTGCGTTAGCATCCGCAGGGACCATCGCAATGCTTTGT124970.17194163477874191No Hit
CTTGTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCC118690.16330121334631414No Hit
AGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGAG117150.16118238388676975No Hit
CGAATGGGGAGCCCACAGGCCGACGCCCGGAGCACGCTGATGCCGAGGCA111630.15358761855126No Hit
GGGAAACTTCGGAGGGAACCAGCTACTAGATGGTTCGATTAGTCTTTCGC111000.15272082468144638No Hit
CTCCTACTCATCGAGGCCTGGCTCTTGCCCCGACGGCCGGGTATAGGTCG110610.1521842380001332No Hit
GTCGAGTTATCATGAATCATCAGAGCAACGGGCAGAGCCCGCGTCGACCT110600.152170479367279No Hit
GCTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACG110110.15149630635742398No Hit
GTCGGCATCGTTTATGGTTGAGACTAGGACGGTATCTGATCGTCTTCGAG108300.14900599381081658No Hit
GCCGGGTATAGGTCGCGCGCTTAAGCGCCATCCATTTTCGGGGCTAGTTG108140.14878585568514965No Hit
CGCGTATTTAAGTCGTCTGCAAAGGATTCTACCCGCCGCTCGGTGGTAAT105700.14542874926872867No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACAGCGCCGGAGGCACGA104020.1431172989492257No Hit
CCCGGATCGACCCGCCGAAGCGAGCCTTGGGACCAAAAACAGGGGTTGTA102870.1415350561709945No Hit
GTCAGTATCGCTGCGGGCTTCCACCAGAGTTTCCTCTGGCTTTACCCCGC102820.14146626300672357No Hit
CTGCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGT94830.13047311535623027No Hit
GTCCGTACCAGTTCTGAGCTGACTGTTCGACGCCCGGGGAAAGCTCCCGA94610.1301704254334382No Hit
GTTAATCATTACTCCGATCCCGAAGGCCAACACAATAGGATCGAAATCCT94090.1294549765250206No Hit
GCCGGAGGCACGACCCGGCCAATTAAGACCAGGAGCGTATCGCCGACCGA93050.12802407870818544No Hit
CTCAAACTTCCTTGGCCTAAACGGCCATAGTCCCTCTAAGAAGCCGGCCG92110.12673076721989213No Hit
GCACGTATTAGCTCTAGAATTACTACGGTTATCCGAGTAGTAGTTACCAT91370.12571262838868247No Hit
CCTCGTTGAAGACCAACAATTGCAATGATCTATCCCCATCACGATGAAAT90190.12408910971188872No Hit
CAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACTAGG88270.12144745220388532No Hit
GCTACTACCACCAAGATCTGCACCGACGGCCGCTCCGCCCGGGCTCGCGC87480.12036052020840476No Hit
CAGAAATTTGAATGATGCGTCGCCAGCACAGAGGCCATGCGATCCGTCGA85190.11720979328479655No Hit
CCGGAATCGAACCCTAATTCTCCGTCACCCGTTACCACCATGGTAGGCCA83100.11433423901827203No Hit
GTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA82990.114182894056876No Hit
GTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGCGCTTACT82760.11386644550122975No Hit
GCGGTGTGTACAAAGGGCAGGGACGTAGTCAACGCGAGCTGATGACTCGC82700.11378389370410465No Hit
GCCGCAGGCTCCACTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTT82550.11357751421129188No Hit
GCAAAGGATTCTACCCGCCGCTCGGTGGTAATTATAATTCAAGGCGGTCC81660.11235299588726946No Hit
CAAAAACAGGGGTTGTACCCCGCCTCCGATTCACGGAGTAAGTAAAATAA81450.11206406459733159No Hit
TTATAATTCAAGGCGGTCCGAACGACGCTTCCGCCGAACGGACTTAGCCA79980.11004154556776649No Hit
CGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCTCGTTAAGGGAT79960.11001402830205813No Hit
GCGCAACTTGCGTTCAAAGACTCGATGGTTCACGGGATTCTGCAATTCAC79420.10927106212793218No Hit
CTTTGACTCTCTTTTCAAAGTCCTTTTCATCTTTACCTCGCGGTACTTGT79270.10906468263511941No Hit
GCCGTTCACATGGAACCTTTCCCCTCTTCGGCCTTCAAAGTTCTCATTTG78900.10855561321951458No Hit
CCACGCTTTCACGGTTCGTATTCGTACTGAAAATCAGAATCAAACGAGCT78440.1079227161082221No Hit
ATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAAGGTGTGAA78420.10789519884251374No Hit
CCAGGCAGACGTGCCCTCGGCCAGAAGGCTTGGGGCGCAACTTGCGTTCA76450.10518474817023941No Hit
GCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAATGGATCCT75170.10342364316490381No Hit
GTTCTAGGTTAGCGCGCAGTTGGGCACCGTAACCCGGCTTCCGGTTCATC73900.1016762967924224No Hit
GCGCGCGTGCGGCCCAGAACATCTAAGGGCATCACAGACCTGTTATTGCC72780.10013532991275376No Hit
CTCCAATGGATCCTCGTTAAGGGATTTAGATTGTACTCATTCCAATTACC72700.10002526084992028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT7050.057.0906434
CCGTACG7450.054.02499833
GTACGTA7600.053.8819635
TATGCCG7050.052.62412348
GTATGCC7250.052.13794347
TACGTAA7950.051.07024436
CGTATGC7900.048.73418446
ATGCCGT7700.047.726349
TCGTATG8500.044.47059645
ACGTCTG9750.042.3572315
GGCAGAC69050.040.3054921
ACGTAAT10150.040.00110637
CTGGCGT5050.038.8201681
CTCGTAT8600.038.66332644
GCAGACG73850.037.728242
CAGACGT76950.036.3452873
AGACGTG78650.035.7377174
GGCGCGC5800.035.611051
CGTCTGA11650.035.4491916
GCCCTCG79800.035.43689710
Produced by FastQC (version 0.11.2)