FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepbur-0_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepbur-0_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23831826
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC598160.2509920977100118TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG107100.056.53824246
CGTATGC114150.053.10983344
GTATGCC115150.052.82955645
ACGTCTG137600.049.59834715
ATGCCGT120900.049.3023547
TCGTATG125500.047.86092843
GCACACG150550.045.30961211
TGCCGTC138100.043.1617848
ACACGTC158250.043.1046113
CACACGT160800.042.44281412
GCCGTCT136250.042.2824249
CTCGTAT120300.039.36742442
CACGTCT175450.039.0783914
AGCACAC178450.038.59810310
CGTCTGA178950.038.29411316
GTCACCT174200.037.97242429
GAGCACA186750.036.9951559
TCGGAAG196150.034.8324853
GATCGGA203900.033.1508331
AGAGCAC211350.032.506968
Produced by FastQC (version 0.11.2)