FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepbur-0_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepbur-0_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13859041
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG28900.046.1430246
GTATGCC34700.039.23845
CGTATGC34100.038.9022544
ACGTCTG47100.033.6610715
ATGCCGT38950.033.4272247
TCGTATG40400.031.363343
ACTTAGG51850.030.03758832
GCACACG54150.029.40840511
CACTTAG55700.028.0244231
CACACGT58350.027.23152412
TGCCGTC47050.027.07737448
TTAGGCA58000.026.97315834
ACACGTC59250.026.8769513
TCACTTA62300.025.50496730
CACGTCT64500.024.79763614
AGTCACT64850.024.44827828
TAGGCAT61650.024.01450735
CTCGTAT44000.023.86526742
AGCACAC69850.023.1489310
CTTAGGC68900.023.01077533
Produced by FastQC (version 0.11.2)