FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepbl-1_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepbl-1_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18226241
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT254550.1396612718991261TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42850.050.7241648
CGTATGC51500.042.34097346
GTATGCC51750.041.797847
CGTGGCC57800.040.20530333
ATGCCGT56850.038.04718449
ACGTGGC65250.035.50763732
TCGTATG61050.034.6862145
CACGTGG69150.034.31501831
GCCTTAT65700.034.25597837
TGCCGTC67200.032.3433150
GTCACGT73600.032.1455129
TCACGTG77350.030.63189130
TATCTCG64100.029.92438141
CTCGTAT64850.029.79322244
GCCGTCT72350.029.5090151
AGTCACG82850.028.89480828
GTGGCCT89150.026.18484334
GGCCTTA89100.025.8869336
ACGTCTG98050.025.02192115
TCTCGTA91650.020.96707243
Produced by FastQC (version 0.11.2)