FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepbl-1_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepbl-1_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21587965
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC297830.1379611278784267TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50150.052.62328746
ACGTCTG103300.044.1810215
GTATGCC63100.042.98856445
CGTATGC62150.040.8302944
GCACACG112500.040.661711
ATGCCGT63600.039.3477147
ACACGTC118250.038.71382513
CACACGT118200.038.6709812
CACTAGC128500.034.9444331
CACGTCT133700.034.3972314
ACTAGCT132300.033.64958632
TCGTATG70550.033.6371643
AGTCACT133200.033.5540628
TATCTCG83550.033.5146139
CGTCTGA136750.033.476116
TGCCGTC74900.032.85058248
AGCACAC140100.032.80092210
TAGCTTA135500.032.673834
TCACTAG138300.032.39233430
GTCACTA139750.031.705729
Produced by FastQC (version 0.11.2)