FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepbl-1_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepbl-1_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17102901
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC189140.11058942573543516TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG32550.048.49574346
GTATGCC42450.038.175245
CGTATGC41550.038.07604244
ATGCCGT43950.036.23476847
ACGTCTG62100.034.66060615
GCACACG63800.033.95677611
CCGATGT61450.033.88833633
CACACGT68450.031.64981312
TGCCGTC51500.030.92268248
ACACGTC72750.029.68288413
TCGTATG51350.029.37827543
TATCTCG55350.028.45602439
GATGTAT76150.026.38197935
CGATGTA81250.025.71638734
CACGTCT85500.025.54295314
GCCGTCT58550.025.46540649
AGCACAC88150.025.17247810
CGTCTGA87150.024.93887516
ACCGATG87750.024.52926332
CTCGTAT59700.024.2134342
Produced by FastQC (version 0.11.2)