FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepakita_hn_r3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepakita_hn_r3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758285
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT290730.1969944339738662TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC48300.057.6070233
TATGCCG48000.055.48947548
GTCCGCA52200.052.83389334
ACGTCCG54900.051.06426632
GTATGCC54250.049.0321647
CGTATGC54850.048.56027246
CCGCACA56500.047.45107736
TCCGCAC60400.045.66154535
CGCACAT57050.045.3379537
ATGCCGT59050.045.2834249
TCGTATG61200.042.60641545
GTCACGT67400.041.54238529
ACGTCTG69300.041.211215
GCACACG71500.040.38398711
TGCCGTC66150.040.1583850
CACACGT75450.038.5014612
GCCGTCT69150.037.9603751
AGTCACG74650.037.64844528
ACACGTC78550.036.71465313
CTCGTAT66250.035.34380344
Produced by FastQC (version 0.11.2)