FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepakita_hn_r2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepakita_hn_r2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26936370
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC774220.2874255142767938TruSeq Adapter, Index 9 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG123800.058.15620446
CGTATGC133450.054.0298144
GTATGCC137000.052.65492645
ATGCCGT142200.050.40851247
ACGTCTG154550.049.1629315
TCGTATG148050.048.56015843
GCACACG158900.047.83988611
TGCCGTC155850.046.12809848
CACACGT167550.045.4949912
GCCGTCT162450.043.88668449
AGTCACG171700.043.19314228
CTCGTAT144450.043.010142
GTCACGA175250.042.19836829
CGATCAG175550.042.12578633
ACACGTC182050.041.87130713
CACGTCT196350.038.71469514
CGTCTGA199500.038.36655416
AGCACAC205850.037.48969710
GAGCACA211250.036.6805539
TCACGAT205650.036.1477130
Produced by FastQC (version 0.11.2)