FastQCFastQC Report
Fri 13 Jul 2018
HH5CFBGX7_n01_nutrinetatrepakita_hn_r1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH5CFBGX7_n01_nutrinetatrepakita_hn_r1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15642000
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC229110.1464710395090142TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45400.053.65714346
CGTATGC50850.048.11369344
GTATGCC54050.045.26457245
ATGCCGT55250.044.09083647
TCGTATG60800.040.0672543
TGCCGTC60550.040.05798348
ACGTCTG63450.039.9911515
GCACACG65900.039.03559511
CACACGT67550.037.92678512
GCCGTCT66150.036.4541449
CTCGTAT60000.033.89290242
ACACGTC76550.033.4678313
CGTCTGA83300.030.79757316
CACGTCT85200.030.35734214
AGCACAC91950.028.775910
GAGCACA97200.027.2935779
TCACGAT87350.026.7660935
TCGGAAG96350.026.738033
GTCACAT102200.024.75967429
GATCGGA103450.024.667751
Produced by FastQC (version 0.11.2)