FastQCFastQC Report
Thu 11 May 2023
HH35LBGXT_n02_mab-3_dmd-3_28.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHH35LBGXT_n02_mab-3_dmd-3_28.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48910119
Sequences flagged as poor quality0
Sequence length36
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6278581.283697551420801No Hit
CGTGCTTGAGCGCGCTGCATACTTGGATCGTCGGAC2058020.4207759134669044No Hit
GCGCGCTGCATACTTGGATCGTCGGACTGTAGAACT1463100.2991405520808485No Hit
GATCGTCGGACTGTAGAACTCTGAACGTGTAGATCT771120.15766062642374679Illumina RNA PCR Primer (100% over 31bp)
CGTGTTTGAGCGCGCTGCATACTTGGATCGTCGGAC577230.11801852291547275No Hit
CGTGCATGAGCGCGCTGCATACTTGGATCGTCGGAC508910.10405004330494472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)